fruK [2018-11-23 17:16:12]

fructose-1-phosphate kinase

locus: BSU14390

pI: 4.89

mw: 32.64 kDa

protein length: 303 aa

gene length: 909 bp

function: fructose utilization

product: fructose-1-phosphate kinase

essential: no

ec: 2.7.1.56

synonyms: fruB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.7|Utilization of fructose]

Gene

Coordinates: 1,508,330 → 1,509,241

The protein

Catalyzed reaction/ biological activity

• ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)

Protein family

• carbohydrate kinase pfkB family (according to Swiss-Prot)

Structure

• [PDB|3OHR]

Expression and Regulation

Operons

• genes: [gene|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|fruR]-[gene|502AA809C8C95F8886DD91FBB7DDBECC63342327|fruK]-[gene|973C3D3FBFDCA095A760C5F49A96D8BE48771014|fruA]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|FruR]: repression, (according to [http://dbtbs.hgc.jp/COG/prom/fruRKA.html DBTBS]), in [regulon|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|FruR regulon]

  regulation

  • induced in the presence of fructose ([protein|7FD2294B0538EB6D83DC3E0752600D2464CDE4A3|FruR]) (according to [http://dbtbs.hgc.jp/COG/prom/fruRKA.html DBTBS])

Biological materials

Mutant

• BKE14390 (Δ[gene|502AA809C8C95F8886DD91FBB7DDBECC63342327|fruK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGGATTAAGTGTTACAGTGT, downstream forward: _UP4_CGTCTATAGAGGAGGAAACA
• BKK14390 (Δ[gene|502AA809C8C95F8886DD91FBB7DDBECC63342327|fruK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGGATTAAGTGTTACAGTGT, downstream forward: _UP4_CGTCTATAGAGGAGGAAACA

References

• 10627040,23033921